Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT546-25UL |
| Description | Anti-Troponin I Antibody, clone 5C11.1 |
| Alternate Names |
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| Background Information | Troponin I, cardiac muscle (UniProt: P19429; also known as Cardiac troponin I) is encoded by the TNNI3 (also known as TNNC1) gene (Gene ID: 7137) in human. Troponin is a complex that regulates the contraction of skeletal muscle, It consists of 3 subunits known as C, T, and I, which are located periodically along the thin filament of the myofibrils. Troponin C (TnC) binds calcium ions, Troponin I is the inhibitory subunit of troponin that inhibits actomyosin ATPase and Troponin T binds to tropomyosin. It also confers calcium-sensitivity to actomyosin ATPase. Amino acids region 32-79 of Troponin I is involved in binding to TnC and region 129-149 is involved in binding to both TnC and actin. It is thought that troponin I acts as a switch, by moving away from actin toward troponin C, following Ca2+ binding to TnC. Cardiac muscle Troponin I has 31 amino acid residues on its N-terminal that are not present in the skeletal muscle form. Hence, cardiac specificity of this isoform is shown to offer accuracy in diagnosis in patients with acute or chronic skeletal muscle injury. Mutation in TNNI3 gene are reported to result in multiple types of cardiomyopathies that can result in premature death. (Ref.: Li, H-C., and Fajer, PG (1998). Biochemistry 37(19); 6628-6635). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Troponin I, clone 5C11.1, Cat. No. MABT546, is a mouse monoclonal antibody that detects Troponin I and has been tested for use in Western Blotting. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Troponin I. |
| Clone | 5C11.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 5C11.1 specifically detects Tropinin I in multiple species. It targets an epitope with in 14 amino acids from the C-terminal region. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Rat, Bovine, Mouse, Chicken based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | ~22 kDa observed; 24.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human skeletal muscle tissue lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Troponin I in human skeletal muscle tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 µL |