Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IF, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT845-25UG |
| Description | Anti-Cytokeratin-20 Antibody, clone XQ1 |
| Alternate Names |
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| Background Information | Keratin, type I cytoskeletal 20 (UniProt: Q9D312; also known as Cytokeratin-20, CK-20, Keratin-20, K20) is encoded by the Krt20 gene (Gene ID: 66809) in murine species. Cytokeratin-20 is type I keratin of the intermediate filament family and is recognized as a marker of intestinal differentiation. It is expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Cytokeratin-20 is also an excellent marker for certain types of carcinomas, such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. It plays a significant role in maintaining keratin filament organization in intestinal epithelia and when phosphorylated, it also plays a role in the secretion of mucin in the small intestine. Cytokeratin-20 can undergo hyperphosphorylation at serine 13 during the early stages of apoptosis, which becomes less prominent during the later stages. Phosphorylation at serine 13 is shown to increase in response to stress brought on by cell injury. During apoptosis it is cleaved at Asp 235 by caspases. (Ref.: Moll, R., et al. (1992). Am. J. Pathol. 140(2); 427-447). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Cytokeratin-20, clone XQ1, Cat. No. MABT845, is a mouse monoclonal antibody that detects Keratin, type I cytoskeletal 20 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A representative lot detected Cytokeratin-20 in Immunofluorescence applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71). Western Blotting Analysis: A representative lot detected Cytokeratin-20 in Western Blotting applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71). |
| Biological Information | |
|---|---|
| Immunogen | A full-length purified murine Cytokeratin-20. |
| Clone | XQ1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone XQ1 is a mouse monoclonal antibody that detects cytoketatin-20 in murine tissues. |
| Isotype | IgMκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. Does not react with human. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Purified by ion-exchange chromatography |
| UniProt Number |
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| Molecular Weight | 49.03 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections. Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Cytokeratin-20 in mouse colon tissue sections. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |