Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, FC, IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABT867-25UG |
| Description | Anti-Podoplanin Antibody, clone LpMab-17 |
| Alternate Names |
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| Background Information | Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-17 is reported to bind to non-PLAG domain and detects endogenous podoplanin in cancer cells and normal cells. It recognizes glycan-deficient podoplanin indicating that its interaction with podoplanin is independent of its glycosylation. The minimum epitope of LpMab-17 is identified as Gly77-Asp82. Although human and monkey podoplanin exhibit 94% homology, this clone does not recognize monkey podoplanin. (Ref.: Kato, Y., et al. (2016). Monoclon. Antib. immunodiag. Immunother. 35(2); 109-116). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Podoplanin, clone LpMab-17, Cat. No. MABT867, is a mouse monoclonal antibody that detects human podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue. Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(2):109-16). Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(2):109-16). Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(2):109-16). ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(2):109-16). |
| Biological Information | |
|---|---|
| Immunogen | LN229 glioma cells expressing human Podoplanin. |
| Clone | LpMab-17 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone LpMab-17 detects podoplanin in human cells. It detects PLAG4 domain. The minimal epitope seunce is shown to be Gly77-Asp82. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 17 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in human colon cancer tissue. Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Podoplanin in human colon cancer tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 25 μg |