| Description | |
|---|---|
| Catalogue Number | MABT911F-25UL |
| Description | Anti-Tspan-33 Antibody, clone 29A6.2 Antibody, FITC conjugate |
| Alternate Names |
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| Background Information | Tetraspanin-33 (UniProt: Q8R3S2; also known as Tspan-33, Penumbra, mPen, Proerythroblast new membrane) is encoded by the Tspan33 (also known as Pen) gene (Gene ID: 232670) in murine species. Tetraspanins are evolutionarily conserved proteins and thus far 33 of them have been described in human and murine species. Tspan-33 is a multi-pass, disulfide-linked homodimeric membrane protein of the tetraspanin (TM4SF) family. It has been reported that Tspan-33 is highly expressed in activated B cells. Its expression has also been reported in malignant B cells. Two isoforms of Tspan-33 have been described that are produced by alternative splicing. Tspan-33 plays an important role in normal erythropoiesis and in the differentiation of erythroid progenitors. Tspan-33 contains four transmembrane domains (aa 25-45, 65-85, 97-117, 236-256), three cytoplasmic domains (aa 1-24, 86-96, 257-283), and two extracellular domains (aa 46-64, 118-235). Several tetraspanins interact with specific membrane proteins via their second extracellular domain (aa 118-235) and incorporate them into tetraspanin-enriched microdomains to modulate the effectiveness of signal transduction by those membrane proteins. (Ref.: Luu, VP., et al. (2013). Clin. Immunol. 149(3): 388-399). |
| Product Information | |
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| Format | FITC |
| Presentation | Purified mouse monoclonal antibody IgM in PBS with 0.1% sodium azide, with 1.5% BSA. |
| Applications | |
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| Application | Anti-Tspan-33, clone 29A6.2, FITC conjugate, Cat. No. MABT911F, is a mouse monoclonal antibody that detects Tetraspanin-33 and has been tested for use in Flow Cytometry. |
| Key Applications |
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| Biological Information | |
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| Immunogen | BaF3 cells expressing full-length murine Tspan-33 fused with a Myc-His tag at the C-terminus. |
| Epitope | extracellular domain |
| Clone | 29A6.2 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 29A6.2 specifically detects Tspan-33 and targets an epitope with in the extracellular domain. |
| Isotype | IgMκ |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 31.55 kDa calculated. |
| Product Usage Statements | |
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| Quality Assurance | Evaluated by Flow Cytometry in mouse splenocytes. Flow Cytometry Analysis: 0.5 µg of this antibody detected Tspan-33 in 500,000 cells per mL mouse splenocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 µL |