We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Share this :
Print
Cell Signaling Skp1 Antibody
Cell Signaling Skp1 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$129.00
Your Price
$129.00
HOW MUCH YOU SAVE:
0.00 %
NETA PART: | CSIG-2156T |
MFG.PART: | 2156T |
UNSPSC: | 12352203 |
Manufacturer: | Cell Signaling |
Size | 20 µl |
Reactivity | H M R |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 19 |
Source/Isotype | Rabbit |
Application/Dilution | {Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:50, Flow Cytometry (Fixed/Permeabilized): 1:200} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | Skp1 Antibody detects endogenous levels of total Skp1 protein. |
Species Reactivity | Human, Mouse, Rat |
Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Skp1. Antibodies are purified by protein A and peptide affinity chromatography. |
Background | Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2 and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggests that activated E2 associates transiently with E3, and the dissociation is a critical step for ubiquitination (5). S phase kinase-associated protein 1 (Skp1) is a critical scaffold protein of the Skp1/CUL1/F-box (SCF) E3 ubiquitin ligase protein complex. Various F-box proteins (e.g., β-TrCP, Skp2) mediate an interaction with Skp1, via their defining and conserved domain of 40 amino acids, and with substrates to be ubiquitinated (e.g., β-catenin, p27) (4). |
SKU | CSIG-2156T |
---|---|
Supplier Part Number | 2156T |
UM | EA |
UNSPSC | 12352203 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |
Hazardous | N |