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Cell Signaling Tcf1/Tcf7 (C63d9) Rabbit mAb
Cell Signaling Tcf1/Tcf7 (C63d9) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
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$151.00
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| NETA PART: | CSIG-2203T |
| MFG.PART: | 2203T |
| UNSPSC: | 12352203 |
| Manufacturer: | Cell Signaling |
| Size | 20 µl |
| Reactivity | H M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 48, 50 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunohistochemistry (Paraffin): 1:50 - 1:200, Immunofluorescence (Frozen): 1:800, Immunofluorescence (Immunocytochemistry): 1:200 - 1:800, Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200, Chromatin IP: 1:50} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #85942 |
| Specificity/Sensitivity | TCF1/TCF7 (C63D9) Rabbit mAb detects endogenous levels of total TCF1/TCF7 protein. This antibody does not recognize the dominant negative isoforms of TCF1/TCF7 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1. TCF1/TCF7 (C63D9) non-specifically labels glomeruli of kidney and fiber-like structures in adipose tissue, skeletal muscle, lung, ovary, colon, and spleen by immunofluorescence. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro96 of human TCF1/TCF7 protein. |
| Background | LEF1 and TCF are members of the high mobility group (HMG) DNA-binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1/TCF7), TCF3/TCF7L1, and TCF4/TCF7L2 (1). LEF1 and TCF1/TCF7 were originally identified as important factors that regulate early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers, including colon cancer (4,5).TCF1/TCF7 has several isoforms due to alternative splicing and transcription from an alternative promoter. The isoforms generated by the alternative promoter do not contain the amino-terminal β-catenin binding domain and therefore may function in a dominant negative manner (6). TCF1/TCF7 displays dynamic expression both in the total amount and the type of isoforms expressed in T cells during development and differentiation (7). |
| SKU | CSIG-2203T |
|---|---|
| Supplier Part Number | 2203T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |
| Hazardous | N |