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Cell Signaling 4e-Bp2 Antibody
Cell Signaling 4e-Bp2 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$129.00
Your Price
$129.00
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NETA PART: | CSIG-2845T |
MFG.PART: | 2845T |
UNSPSC: | 12352203 |
Manufacturer: | Cell Signaling |
Size | 20 µl |
Reactivity | H M R Mk B |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 15 to 20 |
Source/Isotype | Rabbit |
Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunohistochemistry (Paraffin): 1:200 - 1:800} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | 4E-BP2 Antibody detects endogenous levels of total 4E-BP2, independent of phosphorylation. This antibody does not cross-react significantly with 4E-BP1. |
Species Reactivity | Human, Mouse, Rat, Monkey, Bovine |
Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy-terminus of human 4E-BP2. Antibodies are purified by protein A and peptide affinity chromatography. |
Background | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).4E-BP2 and 4E-BP3 share high sequence homology with 4E-BP1, including conservation of the major FRAP/mTOR-dependent phosphorylation sites. Preliminary data suggests that phosphorylation of 4E-BP2 is regulated in a similar manner to that of 4E-BP1, although phosphorylation of this protein has not been as extensively studied (6). |
SKU | CSIG-2845T |
---|---|
Supplier Part Number | 2845T |
UM | EA |
UNSPSC | 12352203 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |