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Cell Signaling Phospho-Smad2 (Ser465/467) (138d4) Rabbit mAb
Cell Signaling Phospho-Smad2 (Ser465/467) (138d4) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
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$156.00
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| NETA PART: | CSIG-3108T |
| MFG.PART: | 3108T |
| UNSPSC: | 12352203 |
| Manufacturer: | Cell Signaling |
| Size | 20 µl |
| Reactivity | H M R Mi |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Simple Western™: 1:50 - 1:250} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-SMAD2 (Ser465/467) (138D4) Rabbit mAb detects endogenous levels of SMAD2 only when dually phosphorylated at serines 465 and 467, and may detect SMAD3 phosphorylated at the equivalent sites. This antibody does not cross-react with other SMAD-related proteins. |
| Species Reactivity | Human, Mouse, Rat, Mink |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser465/467 of human SMAD2. |
| Background | Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8).Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxy-termini (Ser465/467 on Smad2; Ser423/425 on Smad3) by the receptor kinase TGF-β R1 (9-11). Following phosphorylation, Smad2 and Smad3 form a heteromeric complex with the co-Smad family member Smad4. These complexes are translocated to the nucleus where they bind DNA and regulate gene transcription. |
| SKU | CSIG-3108T |
|---|---|
| Supplier Part Number | 3108T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |
| Hazardous | N |