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Cell Signaling Beta-Tubulin (9f3) Rabbit mAb (Alexa Fluor 647 Conjugate)
Cell Signaling Beta-Tubulin (9f3) Rabbit mAb (Alexa Fluor 647 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$435.00
Your Price
$435.00
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NETA PART: | CSIG-3624S |
MFG.PART: | 3624S |
UNSPSC: | 12352203 |
Manufacturer: | Cell Signaling |
Size | 100 µl (50 tests) |
Reactivity | H M R Mk Z B |
Sensitivity | Endogenous |
Source/Isotype | Rabbit IgG |
Application/Dilution | {Immunofluorescence (Immunocytochemistry): 1:50 - 1:200, Flow Cytometry (Fixed/Permeabilized): 1:50} |
Storage | Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
Specificity/Sensitivity | β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) detects endogenous levels of total β-tubulin protein and does not cross-react with recombinant α-tubulin. |
Species Reactivity | Human, Mouse, Rat, Monkey, Zebrafish, Bovine |
Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the amino terminus of human β-tubulin. This antibody was conjugated to Alexa Fluor® 647 under optimal conditions with an F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission with a peak at 665 nm. |
Background | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
SKU | CSIG-3624S |
---|---|
Supplier Part Number | 3624S |
UM | EA |
UNSPSC | 12352203 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | +4C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |