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Cell Signaling Ubc3 Antibody
Cell Signaling Ubc3 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$129.00
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$129.00
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NETA PART: | CSIG-4997T |
MFG.PART: | 4997T |
UNSPSC: | 12352203 |
Manufacturer: | Cell Signaling |
Size | 20 µl |
Reactivity | H M R |
Sensitivity | Endogenous |
Molecular Weight (kDa) | 32 |
Source/Isotype | Rabbit |
Application/Dilution | {Western Blotting: 1:1000} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | UBC3 Antibody detects endogenous levels of total UBC3 and UBC3B protein. |
Species Reactivity | Human, Mouse, Rat |
Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human UBC3. Antibodies are purified by protein A and peptide affinity chromatography. |
Background | Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggest that activated E2 associates transiently with E3, and that the dissociation is a critical step for ubiqitination (5). UBC3, the mammalian orthologue of yeast Cdc34, and UBC3B, a UBC3 family member, are E2 ubiquitin-carrier proteins. These proteins contain a conserved core domain containing a cysteine residue, which forms the thioester bond with ubiquitin (6). UBC3 in concert with the SCFSkp2 (Skp1, Cullin and F-box protein/Skp2) complex mediates cell cycle progression from G1 to S phase by targeting the CDK inhibitor p27 for proteolysis (7). UBC3B in concert with the SCFb-Trcp (Skp1, Cullin and F-box protein/b-Trcp) complex mediates degradation of b-catenin (6). |
SKU | CSIG-4997T |
---|---|
Supplier Part Number | 4997T |
UM | EA |
UNSPSC | 12352203 |
Manufacturer | Cell Signaling |
MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
Temperature | -20C |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |
Hazardous | N |