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Cell Signaling Cd8alpha (Rpa-T8) Mouse mAb (Fitc Conjugate)
Cell Signaling Cd8alpha (Rpa-T8) Mouse mAb (Fitc Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
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$136.00
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$136.00
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| NETA PART: | CSIG-55397S |
| MFG.PART: | 55397S |
| UNSPSC: | 12352203 |
| Manufacturer: | Cell Signaling |
| Size | 500 µl (100 tests) |
| Reactivity | H |
| Sensitivity | Endogenous |
| Source/Isotype | Mouse IgG1 |
| Application/Dilution | {Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600, Flow Cytometry (Fixed/Permeabilized): 1:20, Flow Cytometry (Live): 1:20} |
| Storage | Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | CD8α (RPA-T8) Mouse mAb (FITC Conjugate) recognizes endogenous levels of total CD8α protein. This antibody detects an epitope within the extracellular domain. |
| Species Reactivity | Human |
| Source/Purification | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. |
| Background | Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells and thymocytes (2,3), as well as on certain cell types that do not express the TCR, including some NK cells (4). |
| SKU | CSIG-55397S |
|---|---|
| Supplier Part Number | 55397S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| CountryOfOrigin | US |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |
| Hazardous | N |