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Cell Signaling P44/42 Mapk (Erk1/2) (137f5) Rabbit mAb (Sepharose Bead Conjugate)
Cell Signaling P44/42 Mapk (Erk1/2) (137f5) Rabbit mAb (Sepharose Bead Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
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$435.00
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| NETA PART: | CSIG-5736S |
| MFG.PART: | 5736S |
| UNSPSC: | 12352203 |
| Manufacturer: | Cell Signaling |
| Size | 400 µl |
| Reactivity | H M R Hm Mk Mi Dm Z B Dg Pg Ce |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 42, 44 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Immunoprecipitation: 1:20} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies. |
| Specificity/Sensitivity | p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Sepharose® Bead Conjugate) detects endogenous levels of total p44/42 MAPK (Erk1/2) protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase. The addition of 0.1% SDS to the immunoprecipitation reaction may improve pulldown efficiency. Under some conditions this conjugate may immunoprecipitate p44/Erk1 preferentially over p42/Erk2. |
| Species Reactivity | Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish, Bovine, Dog, Pig, C. elegans |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase. |
| Background | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059. |
| SKU | CSIG-5736S |
|---|---|
| Supplier Part Number | 5736S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |