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Cell Signaling Cd3 (17a2) Rat mAb (Fitc Conjugate)
Cell Signaling Cd3 (17a2) Rat mAb (Fitc Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
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$95.00
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$95.00
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| NETA PART: | CSIG-86603S |
| MFG.PART: | 86603S |
| UNSPSC: | 12352203 |
| Manufacturer: | Cell Signaling |
| Size | 100 µg |
| Reactivity | M |
| Sensitivity | Endogenous |
| Source/Isotype | Rat IgG2b kappa |
| Application/Dilution | {Immunofluorescence (Frozen): 1:800 - 1:1600, Flow Cytometry (Fixed/Permeabilized): 1:100, Flow Cytometry (Live): 1:100} |
| Storage | Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | CD3 (17A2) Rat mAb (FITC Conjugate) recognizes endogenous levels of total CD3ε, CD3γ, and CD3δ proteins. This antibody detects epitopes within the extracellular domains. |
| Species Reactivity | Mouse |
| Source/Purification | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. |
| Background | When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5). |
| SKU | CSIG-86603S |
|---|---|
| Supplier Part Number | 86603S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | +4C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |