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Cell Signaling Mhc Class Ii (I-A/I-E) (M5/114.15.2) Rat mAb (Violetfluor 450 Conjugate)
Cell Signaling Mhc Class Ii (I-A/I-E) (M5/114.15.2) Rat mAb (Violetfluor 450 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
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| NETA PART: | CSIG-86628S |
| MFG.PART: | 86628S |
| UNSPSC: | 12352203 |
| Manufacturer: | Cell Signaling |
| Size | 100 µg |
| Reactivity | M |
| Sensitivity | Endogenous |
| Source/Isotype | Rat IgG2b kappa |
| Application/Dilution | {Flow Cytometry (Fixed/Permeabilized): 1:80, Flow Cytometry (Live): 1:80} |
| Storage | Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | MHC Class II (I-A/I-E) (M5/114.15.2) Rat mAb (violetFluor™ 450 Conjugate) recognizes endogenous levels of total MHC class II (I-A/I-E) proteins. This antibody detects epitopes within the extracellular domain of MHC class II (I-A/I-E). |
| Species Reactivity | Mouse |
| Source/Purification | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. |
| Background | Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells, such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3).The M5/114.15.2 antibody reacts with mouse MHC class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes, but not from mice carrying the H-2s or H-2f haplotypes (4-7). The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, and H-2u haplotypes, but not H-2f, H-2k, or H-2s haplotypes (8,9). |
| SKU | CSIG-86628S |
|---|---|
| Supplier Part Number | 86628S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | +4C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |
| Hazardous | N |