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Cell Signaling Sting (E9x7f) Rabbit mAb
Cell Signaling Sting (E9x7f) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
List Price
$327.00
Your Price
$327.00
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NETA PART: | CSIG-90947S |
MFG.PART: | 90947S |
UNSPSC: | 12352203 |
Manufacturer: | Cell Signaling |
Size | 100 µl |
Reactivity | H M |
Sensitivity | Endogenous |
Source/Isotype | Rabbit IgG |
Application/Dilution | {Immunofluorescence (Frozen): 1:800 - 1:3200, Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800, Flow Cytometry (Fixed/Permeabilized): 1:1600} |
Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
Specificity/Sensitivity | STING (E9X7F) Rabbit mAb recognizes endogenous levels of total STING protein. STING (E9X7F) Rabbit mAb lacks sensitivity in some low-expressing mouse cell lines by immunofluorescence. This lack of sensitivity has not been observed in human cell lines. |
Species Reactivity | Human, Mouse |
Source/Purification | Monoclonal antibody is produced by immunizing animals with human STING recombinant protein and reacts with an epitope near the carboxy terminus. |
Background | Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes and gets phosphorylated by ULK1 at Ser366 (Ser365 in mouse) (7, 8). The TBK1 kinase phosphorylates and activates IRF-3 and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7). |
SKU | CSIG-90947S |
---|---|
Supplier Part Number | 90947S |
UM | EA |
UNSPSC | 12352203 |
Manufacturer | Cell Signaling |
ProductLine | CSIG |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 5 Business Days |
Hazardous | N |