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Cell Signaling Cleaved Drosophila Dcp-1 (Asp216) Antibody
Cell Signaling Cleaved Drosophila Dcp-1 (Asp216) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
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| NETA PART: | CSIG-9578S |
| MFG.PART: | 9578S |
| UNSPSC: | 12352203 |
| Manufacturer: | Cell Signaling |
| Size | 100 µl |
| Reactivity | Dm |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 22 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:200 - 1:800} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Cleaved Drosophila Dcp-1 (Asp215) Antibody recognizes endogenous levels of the large 22 kDa fragment of cleaved Dcp-1. This antibody does not recognize full length Dcp-1. The antibody also detects a non-specific, apoptotic-related band at 50 kDa by western blot. |
| Species Reactivity | D. melanogaster |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp215 of Drosophila Dcp-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Cell death in the fruit fly Drosophila melanogaster is regulated by many of the same stimuli as mammalian cell death (1). The Drosophila genome contains seven caspase genes; three encode initiator caspases, and four encode effector caspases (reviewed in (2)). The Drosophila effector caspase, death caspase-1 (Dcp-1), is a critical executioner of apoptosis. It is involved in the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). The activation of Dcp-1 requires proteolytic processing of its inactive zymogen into active p22 and p13 fragments (3). Comparison of the in vivo activity between DrICE and Dcp-1 has shown that DrICE is a more effective inducer of apoptosis than Dcp-1, which instead plays a role in determining the rate of cell death (4). |
| SKU | CSIG-9578S |
|---|---|
| Supplier Part Number | 9578S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |
| Hazardous | N |