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Key Specifications Table
Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H | IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
Description | |
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Catalogue Number | 09-217 |
Brand Family | Upstate |
Trade Name |
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Description | Anti-Raptor Antibody |
Alternate Names |
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Product Information | |
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Format | Affinity Purified |
Presentation | Affinity purified rabbit polyclonal IgG in 0.02M PBS, 0.25M sodium chloride containing 0.1% sodium azide and 50% glycerol. |
Quality Level | MQ100 |
Applications | |
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Application | Detect Raptor using this anti-Raptor Antibody validated for use in IP & WB. |
Key Applications |
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Biological Information | |
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Immunogen | KLH conjugated peptide encompassing the N-terminal region of human Raptor. |
Host | Rabbit |
Isotype | IgG |
Species Reactivity |
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Species Reactivity Note | Other species have not been tested. |
Antibody Type | Polyclonal Antibody |
Entrez Gene Number |
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Gene Symbol |
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Purification Method | ImmunoAffinity Purified |
UniProt Number |
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UniProt Summary | FUNCTION: SwissProt: Q8N122 # Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions. SIZE: 1335 amino acids; 149038 Da SUBUNIT: Binds directly 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non- phosphorylated form of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Complex with FRAP1 physically interacts under both leucine-rich and -poor conditions and therefore in at least two nutrient-determined states with different stability. TISSUE SPECIFICITY: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. SIMILARITY: SwissProt: Q8N122 ## Contains 7 WD repeats. MISCELLANEOUS: Rapamycin destabilizes the interaction with FRAP1 regardless of nutrient availability, and its potency for dissociation is increased under nutrient-rich conditions. This action uncouples FRAP1 from its substrates, and inhibits FRAP1 signaling without altering its intrinsic catalytic activity. |
Molecular Weight | 150 kDa |
Product Usage Statements | |
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Quality Assurance | Routinely evaluated by immunoblot. |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | 2 years at -20°C from date of shipment |
Packaging Information | |
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Material Size | 100 µL |