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| Description | |
|---|---|
| Catalogue Number | 12-348 |
| Brand Family | Upstate |
| Trade Name |
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| Description | Goat Anti-Rabbit IgG Antibody, HRP-conjugate |
| Background Information | Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Product Information | |
|---|---|
| Format | HRP |
| Presentation | Goat polyclonal conjugated with horseradish peroxidase IgG in buffer containing 50% storage buffer 0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, 0.01% (w/v) gentamicin sulfate. Rehydration: Add 500 µL sterile, distilled water to make a 1 mg/mL stock solution. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Goat Anti-Rabbit IgG Antibody, HRP-conjugate detects level of Rabbit IgG & has been published & validated for use in WB, IH, ELISA. |
| Key Applications |
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| Biological Information | |
|---|---|
| Immunogen | Highly purified whole rabbit IgG. |
| Concentration | 1 mg/mL |
| Host | Goat |
| Specificity | Specific for rabbit IgG heavy and light chains. |
| Isotype | IgG |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by western blot. Western Blot Analysis: Suitable for western blotting (dot blot), ELISA and immunohistochemistry. A working dilution of 1:15,000 to 1:60,000 is suggested for ELISA. A working dilution of 1:2,000 to 1:6,000 is suggested for western blotting procedures. A working dilution of 1:500 to 1:3,000 is suggested for immunohistochemistry. Optimal antibody dilution for other applications should be determined by the researcher. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 500 µg |