Revvity AlphaLISA SureFire Ultra Total ERK 1/2 Assay Kit - 50

Revvity The AlphaLISA SureFire Ultra Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. This kit contains reagents to run 100 wells in 96-well format, using a 80 uL reaction volume. In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal.  The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample. AlphaLISA SureFire Ultra kits are compatible with: Cell and tissue lysates Antibody modulators Biotherapeutic antibodies Alpha SureFire kits can be used for: Cellular kinase assays Receptor activation studies Screening - PEMSD