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Cell Signaling C-Raf (D5x6r) Mouse mAb
Cell Signaling C-Raf (D5x6r) Mouse mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
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$324.00
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| NETA PART: | CSIG-12552S |
| MFG.PART: | 12552S |
| UNSPSC: | 41116133 |
| Manufacturer: | Cell Signaling |
| Size | 100 µl |
| Reactivity | H M R Mk B Pg |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 75 |
| Source/Isotype | Mouse IgG1 |
| Application/Dilution | {Western Blotting: 1:1000} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | c-Raf (D5X6R) Mouse mAb recognizes endogenous levels of total c-Raf protein. This antibody does not cross-react with A-Raf or B-Raf. |
| Species Reactivity | Human, Mouse, Rat, Monkey, Bovine, Pig |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to residues in the middle of human c-Raf protein. |
| Background | A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites, including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499 (2). p21-activated kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events (11). |
| SKU | CSIG-12552S |
|---|---|
| Supplier Part Number | 12552S |
| UM | UNIT |
| UNSPSC | 41116133 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |