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Genscript Enterokinase Animal-Free, His, Bovine
NETA PART: | GSCRPT-Z03747-100 |
MFG.PART: | Z03747-100 |
UNSPSC: | 12352204 |
Manufacturer: | GenScript USA Inc |
Enterokinase (EK) is a serine protease producedby cells of the duodenum, and is involved in mammalian digestion. It plays the roleof turning trypsinogen to the active form of trypsin, and indirectly activatesthe pancreatic digestive enzymes. Enterokinase is a specific protease thatcleaves after a lysine at its cleavage site: Asp-Asp-Asp-Asp-Lys. Enterokinase doesnot function if the cleavage site is followed by a proline. |
Source | Recombinant Enterokinase expressed in P. pastoris |
Species | Bovine |
Tag | His Tag |
Molecular Weight | Predicted MW: 27 kDa Apparent MW: ~40 kDa, on SDS-PAGE under reducingconditions |
Purity | ≥ 95% as analyzed by SDS-PAGE |
Endotoxin Level | < 1.0 EU/μg of protein by gel clotting method |
Biological Activity | 5 U/μl Unit definition: One unit is defined as the amount of enzyme sufficientto cleave 50 μg of control fusion protein (~24 kDa) in 16 hours to 95%completion at 22 °C in a buffer containing 20 mM Tris-HCl, 50 mMNaCl, 2 mM CaCl2, pH 7.4. |
Formulation | Supplied as solution in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2,50% glycerol, pH 7.4. |
Storage Buffer | 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50% glycerol, pH7.4. |
Storage & Stability | Uponreceiving, the product remains stable for up to 6 months at -20 °C. Thisproduct is stable for up to 1 week at 37 °C. Avoid repeated freeze-thaw cyclesby making single-use aliquots before the solution is stored at -20 °C. |
For laboratory research or manufacture use only. Direct humanuse, including taking orally and injection and clinical use are forbidden.
SKU | GSCRPT-Z03747-100 |
---|---|
Supplier Part Number | Z03747-100 |
UM | UN |
UNSPSC | 12352204 |
Manufacturer | GenScript USA Inc |
CountryOfOrigin | CN |
ProductLine | GSCRPT |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 7 Business Days |