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Beckman Annexin V-Fitc Kit, 20 Tests, Ruo
Beckman Annexin V Fitc Kit 20 Test, Ruo - BKMPFZ (Additional S&H Or Hazmat Fees May Apply)
List Price
$122.40
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$122.40
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NETA PART: | BKMPFZ-IM2375 |
MFG.PART: | IM2375 |
UNSPSC: | 41116001 |
Manufacturer: | Beckman Coulter |
Beckman in early apoptosis, cells lose membrane phospholipid asymmetry, whilst the integrity of the cell membrane is maintained. the annexin-v kits contain a viability dye to evaluate membrane integrity as well as annexin-v to assess membrane asymmetry. in fact, phosphatidylserine (ps), a negatively charged phospholipid located in the inner surface of the plasma membrane, appears very early in apoptosis at the external cell surface. annexin v, a calcium and phospholipid binding protein, binds preferentially to ps. the annexin v kits employ recombinant annexin v labeled with biotin, fluorescein or r-phycoerythrin in together with the viability dyes propidium iodide (pi) or 7-amino actinomycin d (7-aad). apoptotic cells are easily identified utilizing a rapid and simple staining procedure. fixation of the cells is not necessary. when cells are double-stained with annexin v and a viability dye, three different cell populations may be observed (see figure below): (i) live cells that do not stain either with annexin v or with the vital dye, (ii) necrotic or late apoptotic cells that stain with both annexin v and the vital dye, and (iii) early apoptotic cells that stain with annexin v only. analysis may be performed on any flow cytometer equipped with a 488nm laser or arc lamp depending on the reagent kit chosen. alternatively, the cells may be examined with a fluorescent microscope or cell imaging system equipped with the appropriate filters. - BKMPFZ (Additional S&H Or Hazmat Fees May Apply)
SKU | BKMPFZ-IM2375 |
---|---|
Supplier Part Number | IM2375 |
UM | UNIT |
UNSPSC | 41116001 |
Manufacturer | Beckman Coulter |
ProductLine | BKMPFZ |
Qty | 1 |
MinOrderQty | 1 |
Weight | 7.00 |
Lead Time | 21 Business Days |
Hazardous | N |