Beckman Annexin V-Fitc Kit, 200 Tests, Ruo

Beckman in early apoptosis, cells lose membrane phospholipid asymmetry, whilst the integrity of the cell membrane is maintained. the annexin-v kits contain a viability dye to evaluate membrane integrity as well as annexin-v to assess membrane asymmetry. in fact, phosphatidylserine (ps), a negatively charged phospholipid located in the inner surface of the plasma membrane, appears very early in apoptosis at the external cell surface. annexin v, a calcium and phospholipid binding protein, binds preferentially to ps. the annexin v kits employ recombinant annexin v labeled with biotin, fluorescein or r-phycoerythrin in together with the viability dyes propidium iodide (pi) or 7-amino actinomycin d (7-aad). apoptotic cells are easily identified utilizing a rapid and simple staining procedure. fixation of the cells is not necessary. when cells are double-stained with annexin v and a viability dye, three different cell populations may be observed (see figure below): (i) live cells that do not stain either with annexin v or with the vital dye, (ii) necrotic or late apoptotic cells that stain with both annexin v and the vital dye, and (iii) early apoptotic cells that stain with annexin v only. analysis may be performed on any flow cytometer equipped with a 488nm laser or arc lamp depending on the reagent kit chosen. alternatively, the cells may be examined with a fluorescent microscope or cell imaging system equipped with the appropriate filters. - BKMPFZ (Additional S&H Or Hazmat Fees May Apply)