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LI-COR IRDye® 800cw Donkey Anti-Guinea Pig Igg Secondary Antibody, 0.5 mg
NETA PART: | LIC-926-32411 |
MFG.PART: | 926-32411 |
UNSPSC: | 12352203 |
Manufacturer: | LI-COR BioScience |
Immunogen
Guinea Pig IgG, whole molecule
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule guinea pig IgG as well as the light chains common to other guinea pig immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ Assay applications.
Applications
Highly recommended for:
- Western Blot
- In-Cell Western Assay
- On-Cell Western Assay
- Protein Array
- Immunohistochemistry
- Small Animal Imaging
- Microscopy
- 2D Gel Detection
- Tissue Section Imaging
- Virus Titration Assay
Formulation
IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
Recommended Dilutions
Application | Recommended | Suggested Range |
---|---|---|
Odyssey Western blot detection | 1:20,000 | 1:5,000 - 1:25,000 |
In-Cell Western Assay detection | 1:800 | 1:200 - 1:1,200 |
Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
RRID
- P/N 925-32411: RRID AB_2814905
- P/N 926-32411: RRID AB_1850024
Example Data
SKU | LIC-926-32411 |
---|---|
Supplier Part Number | 926-32411 |
UM | EA |
UNSPSC | 12352203 |
Manufacturer | LI-COR BioScience |
CountryOfOrigin | US |
ProductLine | LIC |
Qty | 1 |
MinOrderQty | 1 |
Weight | 10.00 |
Lead Time | 5 Business Days |