Revvity Alphalisa Surefire Ultra Total Vegfr-2 Control

Revvity This Control Lysate Is Intended For Use With The Alphalisa Surefire Ultra Total Vegfr-2 Assay Kit, Or For Use As A Control For Those Running Orthogonal Or Secondary Assays Along With The Surefire Assay. To Prepare The Lysate, Huvec Cells Were Grown To Confluence In T175 Flasks, And Then Serum-Starved Overnight. The Following Day The Media Was Removed, And The Cells Were Treated With 100 Ng/Ml Vegf For 10 Minutes. The Agonist Was Removed, And The Cells Were Lysed With 4 Ml Of Freshly Prepared 1x Lysis Buffer, With Shaking For 10 Minutes.S - PEGSK (Additional S&H Or Hazmat Fees May Apply)