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| Description | |
|---|---|
| Catalogue Number | 14-865 |
| Brand Family | Upstate |
| Trade Name |
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| Description | p53-GST (recombinant protein) |
| Overview | N-terminal GST-tagged, recombinant full length human P53 protein, expressed in E. coli. Purified using glutathione agarose chromatography. |
| Alternate Names |
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| Product Information | |
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| Presentation | Recombinant protein in buffer containing 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 5mM glutathione, 0.5 mM EDTA, 0.5 mM DTT, 1 mM PMSF containing 20% glycerol. |
| Quality Level | MQ100 |
| Applications | |
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| Key Applications |
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| Biological Information | |
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| Concentration | 0.5 mg/mL |
| Purity | ~95% by SDS-PAGE |
| Source | Full-length human p53 protein, expressed in e. coli. |
| Specific Activity | For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where its believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq] |
| Gene Symbol |
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| Purification Method | Glutathione agarose chromatography |
| UniProt Number |
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| Molecular Weight | 72 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by SDS-PAGE and Coomassie Stain |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | 1 year at -20°C |
| Packaging Information | |
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| Material Size | 50 µg |