Takara PCR Human Papillomavirus Typing Set \50 Rxns

Takara This is a primer set designed to identify a broad range of human papillomavirus (HPV) types by PCR. This set utilizes two pairs of consensus primers, designed for amplifying the sequence of HPV homologous region containing the E6 and E7 regions of HPV. The malignant HPV 16, 18, 33, 52b and 58 are amplified using the HPVpU-1M/HPVpU-2R primer pair; the benign HPV 6 and 11 are amplified using the HPVpU-31B/HPVpU-2R primer pair. Through restrcition enzyme digestion (AccI, AfaI, AvaI, AvaII and BglII, available separately as Enzyme Set A (Cat.# 6604)) and subsequent agarose gel electrophoresis, HPV types can be identified. PCR reagents need to be purchased separately. This primer set is optimized for use with Takara Taq. - TAKBGN (Additional S&H or Hazmat Fees May Apply)