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Cell Signaling Phospho-4e-Bp1 (Ser65) (D9g1q) Rabbit mAb
Cell Signaling Phospho-4e-Bp1 (Ser65) (D9g1q) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
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$387.00
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$387.00
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| NETA PART: | CSIG-13443S |
| MFG.PART: | 13443S |
| UNSPSC: | 41105803 |
| Manufacturer: | Cell Signaling |
| Size | 100 µl |
| Reactivity | H Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 15-20 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-4E-BP1 (Ser65) (D9G1Q) Rabbit mAb recognizes endogenous levels of 4E-BP1 protein only when phosphorylated at Ser65. |
| Species Reactivity | Human, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser65 of human 4E-BP1 protein. |
| Background | Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5). |
| SKU | CSIG-13443S |
|---|---|
| Supplier Part Number | 13443S |
| UM | UNIT |
| UNSPSC | 41105803 |
| Manufacturer | Cell Signaling |
| MSDS URL | https://www.cellsignal.com/contents/technical/safety-data-sheet-(sds)/resources-safety-data-sheets |
| Temperature | -20C |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.00 |
| Lead Time | 5 Business Days |